Command Line Interface

pyprophet is the main command-line interface for PyProphet, with subcommands for scoring, IPF, levels context inference, and other utility functions.

pyprophet

PyProphet: Semi-supervised learning and scoring of OpenSWATH results.

Visit http://openswath.org for usage instructions and help.

pyprophet [OPTIONS] COMMAND [ARGS]...

Options

--version: Show the version and exit.

--log-level <log_level>

Set global logging level.

Options:: TRACE | DEBUG | INFO | SUCCESS | WARNING | ERROR | CRITICAL

--log-colorize, --no-log-colorize: Turn on/off colorized logging output.

--helphelp: Show advanced help with all options.

Semi-Supervised Scoring of Peak-Groups

PyProphet provides a command-line interface for scoring peak-groups using the score subcommand. This provides a re-implementation of the original mProphet algorithm, which is a semi-supervised machine learning approach for scoring peak-groups in SRM mass spectrometry data.

pyprophet score

Conduct semi-supervised learning and error-rate estimation for MS1, MS2 and transition-level data.

Note

When using –classifier HistGradientBoosting, the OMP_NUM_THREADS environment variable controls OpenMP thread usage to avoid CPU oversubscription. The CLI will automatically set it if not already specified, but for best control and performance, set it explicitly before launching pyprophet:

For example, if your machine has 20 CPU threads and you want to use 3 threads for semi-supervised learning, set OMP_NUM_THREADS to 7 (ceil(20/3)):

Example

export OMP_NUM_THREADS=7
pyprophet score --in input.osw --classifier HistGradientBoosting --threads 3

# Or in one line (automatic setting):
pyprophet score --in input.osw --classifier HistGradientBoosting --threads 3

pyprophet score [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file. Valid formats are .osw, .parquet and .tsv.

--out <outfile>: PyProphet output file. Valid formats are .osw, .parquet and .tsv. Must be the same format as input file.

--subsample_ratio <subsample_ratio>

Subsampling ratio for large data. Use <1.0 to subsample precursors for semi-supervised learning, the learned weights will then be applied to the full data set. When set to 1.0 (default) and the input has >20 runs, auto-subsampling to 1/N is applied (N=number of runs). Set to -1.0 to disable auto-subsampling and use full data.

Default:: 1.0

--classifier <classifier>

Either a “LDA”, “SVM”, “XGBoost” or “HistGradientBoosting” classifier is used for semi-supervised learning.

Default:: 'LDA'
Options:: LDA | SVM | XGBoost | HistGradientBoosting

--apply_weights <apply_weights>: Apply PyProphet score weights file (.csv/.bin) instead of semi-supervised learning.

--xeval_num_iter <xeval_num_iter>

Number of iterations for cross-validation of semi-supervised learning step.

Default:: 10

--ss_num_iter <ss_num_iter>

Number of iterations for semi-supervised learning step.

Default:: 10

--ss_scale_features, --no-ss_scale_features

Scale / standardize features to unit variance before semi-supervised learning.

Default:: False

--parametric, --no-parametric

Do parametric estimation of p-values.

Default:: False

--level <level>

Either “ms1”, “ms2”, “ms1ms2”, “transition”, or “alignment”; the data level selected for scoring. “ms1ms2 integrates both MS1- and MS2-level scores and can be used instead of “ms2”-level results.”

Default:: 'ms2'
Options:: ms1 | ms2 | ms1ms2 | transition | alignment

--threads <threads>

Number of threads used for semi-supervised learning. -1 means all available CPUs.

Default:: 1

--profile: Enable memory allocation tracking and profiling. Requires memrary to be installed.

The score command has several advanced options that can be seen using the --helphelp flag.

Inference of Peptidoforms

For PTM analyses, PyProphet provides the infer peptidoform subcommand. This command allows you to perform inference of peptidoforms, for site-localization of peptidoforms in large-scale DIA experiments.

Refer to Rosenberger, G. et. al. (2017) to learn more about the inference of peptidoforms workflow.

pyprophet infer peptidoform

Infer peptidoforms after scoring of MS1, MS2 and transition-level data.

pyprophet infer peptidoform [OPTIONS]

Options

--in <infile>: Required PyProphet input file. Valid formats are .osw, .parquet (produced by export parquet)

--out <outfile>: PyProphet output file. Valid formats are .osw, .parquet. Must be the same format as input file.

--ipf_ms1_scoring, --no-ipf_ms1_scoring

Use MS1 precursor data for IPF.

Default:: True

--ipf_ms2_scoring, --no-ipf_ms2_scoring

Use MS2 precursor data for IPF.

Default:: True

--ipf_h0, --no-ipf_h0

Include possibility that peak groups are not covered by peptidoform space.

Default:: True

--ipf_grouped_fdr, --no-ipf_grouped_fdr

[Experimental] Compute grouped FDR instead of pooled FDR to better support data where peak groups are evaluated to originate from very heterogeneous numbers of peptidoforms.

Default:: False

--ipf_max_precursor_pep <ipf_max_precursor_pep>

Maximum PEP to consider scored precursors in IPF.

Default:: 0.7

--ipf_max_peakgroup_pep <ipf_max_peakgroup_pep>

Maximum PEP to consider scored peak groups in IPF.

Default:: 0.7

--ipf_max_precursor_peakgroup_pep <ipf_max_precursor_peakgroup_pep>

Maximum BHM layer 1 integrated precursor peakgroup PEP to consider in IPF.

Default:: 0.4

--ipf_max_transition_pep <ipf_max_transition_pep>

Maximum PEP to consider scored transitions in IPF.

Default:: 0.6

--propagate_signal_across_runs, --no-propagate_signal_across_runs

Propagate signal across runs (requires running alignment).

Default:: False

--ipf_max_alignment_pep <ipf_max_alignment_pep>

Maximum PEP to consider for good alignments.

Default:: 1.0

--across_run_confidence_threshold <across_run_confidence_threshold>

Maximum PEP to consider for propagating signal across runs for aligned features.

Default:: 0.5

For glycoform inference, you can use the infer glycoform subcommand, which is specifically designed for glycopeptide analyses.

pyprophet infer glycoform

Infer glycoforms after scoring of MS1, MS2 and transition-level data.

pyprophet infer glycoform [OPTIONS]

Options

--in <infile>: Required Input file.

--out <outfile>: Output file.

--ms1_precursor_scoring, --no-ms1_precursor_scoring

Use MS1 precursor data for glycoform inference.

Default:: True

--ms2_precursor_scoring, --no-ms2_precursor_scoring

Use MS2 precursor data for glycoform inference.

Default:: True

--grouped_fdr, --no-grouped_fdr

[Experimental] Compute grouped FDR instead of pooled FDR to better support data where peak groups are evaluated to originate from very heterogeneous numbers of glycoforms.

Default:: False

--max_precursor_pep <max_precursor_pep>

Maximum PEP to consider scored precursors.

Default:: 1

--max_peakgroup_pep <max_peakgroup_pep>

Maximum PEP to consider scored peak groups.

Default:: 0.7

--max_precursor_peakgroup_pep <max_precursor_peakgroup_pep>

Maximum BHM layer 1 integrated precursor peakgroup PEP to consider.

Default:: 1

--max_transition_pep <max_transition_pep>

Maximum PEP to consider scored transitions.

Default:: 0.6

--use_glycan_composition, --use_glycan_struct

Compute glycoform-level FDR based on glycan composition or struct.

Default:: True

--ms1_mz_window <ms1_mz_window>

MS1 m/z window in Thomson or ppm.

Default:: 10

--ms1_mz_window_unit <ms1_mz_window_unit>

MS1 m/z window unit.

Default:: 'ppm'
Options:: ppm | Da | Th

--propagate_signal_across_runs, --no-propagate_signal_across_runs

Propagate signal across runs (requires running alignment).

Default:: False

--max_alignment_pep <max_alignment_pep>

Maximum PEP to consider for good alignments.

Default:: 1.0

--across_run_confidence_threshold <across_run_confidence_threshold>

Maximum PEP to consider for propagating signal across runs for aligned features.

Default:: 0.5

Refer to Yang, Y. et. al. (2021) to learn more about the glycoform inference workflow.

Peptide / Protein / Gene Inference

To perform inference at different levels of biological context and different experimental contexts (global, experiment-wide and run-specific), PyProphet provides the infer subcommand. This command allows you to infer peptide, glycopeptide, protein, and gene levels from your data.

Refer to Rosenberger, G. et. al. (2017) to learn more about the levels context inference.

For more information about glycopeptide inference, refer to Yang, Y.. et. al. (2021).

pyprophet infer peptide

Infer peptides and conduct error-rate estimation in different contexts.

pyprophet infer peptide [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file. Valid formats are .osw, .parquet (produced by export parquet)

--out <outfile>: PyProphet output file. Valid formats are .osw, .parquet. Must be the same format as input file.

--context <context>

Context to estimate peptide-level FDR control.

Default:: 'run-specific'
Options:: run-specific | experiment-wide | global

--parametric, --no-parametric

Do parametric estimation of p-values.

Default:: False

--color_palette <color_palette>

Color palette to use in reports.

Default:: 'normal'
Options:: normal | protan | deutran | tritan

The peptide command accepts a helphelp argument to display its advanced options that are not shown here.

pyprophet infer glycopeptide

Infer glycopeptides and conduct error-rate estimation in different contexts.

pyprophet infer glycopeptide [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required Input file.

--out <outfile>: Output file.

--context <context>

Context to estimate glycopeptide-level FDR control.

Default:: 'run-specific'
Options:: run-specific | experiment-wide | global

--density_estimator <density_estimator>

Either kernel density estimation (“kde”) or Gaussian mixture model (“gmm”) is used for score density estimation.

Default:: 'gmm'
Options:: kde | gmm

--grid_size <grid_size>

Number of d-score cutoffs to build grid coordinates for local FDR calculation.

Default:: 256

--parametric, --no-parametric

Do parametric estimation of p-values.

Default:: False

pyprophet infer protein

Infer proteins and conduct error-rate estimation in different contexts.

pyprophet infer protein [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file. Valid formats are .osw, .parquet (produced by export parquet)

--out <outfile>: PyProphet output file. Valid formats are .osw, .parquet. Must be the same format as input file.

--context <context>

Context to estimate protein-level FDR control.

Default:: 'run-specific'
Options:: run-specific | experiment-wide | global

--parametric, --no-parametric

Do parametric estimation of p-values.

Default:: False

--color_palette <color_palette>

Color palette to use in reports.

Default:: 'normal'
Options:: normal | protan | deutran | tritan

The protein command accepts a helphelp argument to display its advanced options that are not shown here.

pyprophet infer gene

Infer genes and conduct error-rate estimation in different contexts.

pyprophet infer gene [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file. Valid formats are .osw, .parquet (produced by export parquet)

--out <outfile>: PyProphet output file. Valid formats are .osw, .parquet. Must be the same format as input file.

--context <context>

Context to estimate gene-level FDR control.

Default:: 'run-specific'
Options:: run-specific | experiment-wide | global

--parametric, --no-parametric

Do parametric estimation of p-values.

Default:: False

--color_palette <color_palette>

Color palette to use in reports.

Default:: 'normal'
Options:: normal | protan | deutran | tritan

The gene command accepts a helphelp argument to display its advanced options that are not shown here.

Exporters

PyProphet provides several export utilities to export between different file formats for OpenSwath’s (.osw / *.sqMass*sqlite-based formats) and experimental parquet formats, as well as exporting PDF reports of the data.

TSV Results (Proteomics)

To export results from a post-scoring workflow (using the .osw input workflow) to a tab-separated values (TSV) file, you can use the export tsv subcommand. This is useful for exporting results in a format that can be easily read and processed by other tools or scripts.

pyprophet export tsv

Export Proteomics/Peptidoform TSV/CSV tables

pyprophet export tsv [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file.

--out <outfile>: Output TSV/CSV (legacy_split, legacy_merged) file.

--format <format>

Export format, either legacy_split/legacy_merged (mProphet/PyProphet).

Default:: 'legacy_merged'
Options:: legacy_split | legacy_merged

--csv, --no-csv

Export CSV instead of TSV file.

Default:: False

--transition_quantification, --no-transition_quantification

[format: legacy] Report aggregated transition-level quantification.

Default:: True

--max_transition_pep <max_transition_pep>

[format: legacy] Maximum PEP to retain scored transitions for quantification (requires transition-level scoring).

Default:: 0.7

--ipf <ipf>

[format: matrix/legacy] Should IPF results be reported if present? “peptidoform”: Report results on peptidoform-level, “augmented”: Augment OpenSWATH results with IPF scores, “disable”: Ignore IPF results

Default:: 'peptidoform'
Options:: peptidoform | augmented | disable

--ipf_max_peptidoform_pep <ipf_max_peptidoform_pep>

[format: matrix/legacy] IPF: Filter results to maximum run-specific peptidoform-level PEP.

Default:: 0.4

--max_rs_peakgroup_qvalue <max_rs_peakgroup_qvalue>

[format: matrix/legacy] Filter results to maximum run-specific peak group-level q-value.

Default:: 0.05

--peptide, --no-peptide

Append peptide-level error-rate estimates if available.

Default:: True

--max_global_peptide_qvalue <max_global_peptide_qvalue>

[format: matrix/legacy] Filter results to maximum global peptide-level q-value.

Default:: 0.01

--protein, --no-protein

Append protein-level error-rate estimates if available.

Default:: True

--max_global_protein_qvalue <max_global_protein_qvalue>

[format: matrix/legacy] Filter results to maximum global protein-level q-value.

Default:: 0.01

--use_alignment, --no-use_alignment

Use alignment results to recover peaks with good alignment scores if alignment data is present in the input file.

Default:: True

--max_alignment_pep <max_alignment_pep>

[format: matrix/legacy] Maximum PEP to consider for good alignments when use_alignment is enabled.

Default:: 0.7

--exclude-decoys, --no-exclude-decoys

Exclude decoy entries from the exported results. Use –no-exclude-decoys to retain decoys.

Default:: True

TSV Results (Small Molecules)

This is similar to the TSV export for proteomics, but specifically designed for small molecule data. It allows you to export results in a tab-separated values (TSV) format, which can be easily read and processed by other tools or scripts.

pyprophet export compound

Export Compound TSV/CSV tables

pyprophet export compound [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file.

--out <outfile>: Output TSV/CSV (matrix, legacy_merged) file.

--format <format>

Export format, either matrix, legacy_merged (PyProphet) or score_plots format.

Default:: 'legacy_merged'
Options:: matrix | legacy_merged

--csv, --no-csv

Export CSV instead of TSV file.

Default:: False

--max_rs_peakgroup_qvalue <max_rs_peakgroup_qvalue>

[format: matrix/legacy] Filter results to maximum run-specific peak group-level q-value.

Default:: 0.05

TSV Results (Glycoform)

This is similar to the TSV export for proteomics, but specifically designed for glycoform data. It allows you to export results in a tab-separated values (TSV) format, which can be easily read and processed by other tools or scripts.

pyprophet export glyco

Export Gylcoform TSV/CSV tables

pyprophet export glyco [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file.

--out <outfile>: Output TSV/CSV (matrix, legacy_split, legacy_merged) file.

--format <format>

Export format, either matrix, legacy_split/legacy_merged (mProphet/PyProphet) format.

Default:: 'legacy_split'
Options:: matrix | legacy_split | legacy_merged

--csv, --no-csv

Export CSV instead of TSV file.

Default:: False

--transition_quantification, --no-transition_quantification

[format: legacy] Report aggregated transition-level quantification.

Default:: True

--max_transition_pep <max_transition_pep>

[format: legacy] Maximum PEP to retain scored transitions for quantification (requires transition-level scoring).

Default:: 0.7

--max_rs_peakgroup_qvalue <max_rs_peakgroup_qvalue>

[format: matrix/legacy] Filter results to maximum run-specific peak group-level q-value.

Default:: 0.05

--glycoform_match_precursor <glycoform_match_precursor>

[format: matrix/legacy] Export glycoform results with glycan matched with precursor-level results.

Default:: 'glycan_composition'
Options:: exact | glycan_composition | none

--max_glycoform_pep <max_glycoform_pep>

[format: matrix/legacy] Filter results to maximum glycoform PEP.

Default:: 1

--max_glycoform_qvalue <max_glycoform_qvalue>

[format: matrix/legacy] Filter results to maximum glycoform q-value.

Default:: 0.05

--glycopeptide, --no-glycopeptide

Append glycopeptide-level error-rate estimates if available.

Default:: True

--max_global_glycopeptide_qvalue <max_global_glycopeptide_qvalue>

[format: matrix/legacy] Filter results to maximum global glycopeptide-level q-value.

Default:: 0.01

TSV Quantification Matrices (Proteomics)

To export quantification matrices from a post-scoring workflow to a tab-separated values (TSV) file, you can use the export matrix subcommand. This is useful for exporting quantification data in a format that can be easily read and processed by other tools or scripts.

pyprophet export matrix

Export Proteomics/Peptidoform Quantification Matrix

pyprophet export matrix [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file.

--out <outfile>: Output TSV/CSV file.

--level <level>

Export quantification level, either precursor, peptide, protein, or gene.

Default:: 'peptide'
Options:: precursor | peptide | protein | gene

--csv, --no-csv

Export CSV instead of TSV file.

Default:: False

--transition_quantification, --no-transition_quantification

[format: legacy] Report aggregated transition-level quantification.

Default:: True

--max_transition_pep <max_transition_pep>

[format: legacy] Maximum PEP to retain scored transitions for quantification (requires transition-level scoring).

Default:: 0.7

--ipf <ipf>

[format: matrix/legacy] Should IPF results be reported if present? “peptidoform”: Report results on peptidoform-level, “augmented”: Augment OpenSWATH results with IPF scores, “disable”: Ignore IPF results

Default:: 'peptidoform'
Options:: peptidoform | augmented | disable

--ipf_max_peptidoform_pep <ipf_max_peptidoform_pep>

[format: matrix/legacy] IPF: Filter results to maximum run-specific peptidoform-level PEP.

Default:: 0.4

--max_rs_peakgroup_qvalue <max_rs_peakgroup_qvalue>

[format: matrix/legacy] Filter results to maximum run-specific peak group-level q-value.

Default:: 0.05

--max_global_peptide_qvalue <max_global_peptide_qvalue>

[format: matrix/legacy] Filter results to maximum global peptide-level q-value.

Default:: 0.01

--max_global_protein_qvalue <max_global_protein_qvalue>

[format: matrix/legacy] Filter results to maximum global protein-level q-value.

Default:: 0.01

--use_alignment, --no-use_alignment

Use alignment results to recover peaks with good alignment scores if alignment data is present in the input file.

Default:: True

--max_alignment_pep <max_alignment_pep>

[format: matrix/legacy] Maximum PEP to consider for good alignments when use_alignment is enabled.

Default:: 0.7

--top_n <top_n>

[format: matrix/legacy] Number of top intense features to use for summarization

Default:: 3

--consistent_top, --no-consistent_top

[format: matrix/legacy] Whether to use same top features across all runs

Default:: True

--normalization <normalization>

[format: matrix/legacy] Normalization method to apply to the quantification matrix.

Default:: 'none'
Options:: none | median | medianmedian | quantile

--exclude-decoys, --no-exclude-decoys

Exclude decoy entries from the exported matrix. Use –no-exclude-decoys to retain decoys.

Default:: True

Convert OSW to Parquet

To convert OpenSwath’s .osw / .sqMass format to a parquet format, you can use the export parquet subcommand. This is useful for converting results from the .osw / .sqMass format to a more efficient and space saving data storage format. This subcommand has the option to convert the entire .osw file to a snigle parquet file (with both precursor and transition data) or to split the parquet file into a separate precursors_features.parquet file and a transition_features.parquet file. There is the option to further split by run, which is useful for large datasets.

pyprophet export parquet

Export OSW or sqMass to parquet format

pyprophet export parquet [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet OSW or sqMass input file.

--out <outfile>: Output parquet file.

--pqpfile <pqpfile>: PyProphet PQP file. Only required when converting sqMass to parquet.

--transitionLevel: Whether to export transition level data as well

--onlyFeatures: Only include precursors that have a corresponding feature

--noDecoys: Do not include decoys in the exported data

--split_transition_data, --no-split_transition_data

Split transition data into a separate parquet (default: True).

Default:: False

--split_runs, --no-split_runs

Split runs into separate parquet files/directories (default: False).

Default:: False

--compression <compression>

Compression algorithm to use for parquet file.

Default:: 'zstd'
Options:: lz4 | uncompressed | snappy | gzip | lzo | brotli | zstd

--compression_level <compression_level>

Compression level to use for parquet file.

Default:: 11

--include_transition_data, --no-include_transition_data

Include transition data in the exported parquet file(s). When disabled, only precursor-level data is exported.

Default:: True

Export Feature Score Plots

To export the distribution of feature scores (VAR_ columns) and, if available, scorer scores (SCORE columns), you can use the export feature-scores subcommand. This command works with all file formats (OSW, Parquet, and Split Parquet):

For unscored files: Plots only VAR_ columns (feature variables)
For scored files: Applies RANK==1 filtering and plots both SCORE and VAR_ columns

This is useful for investigating the distribution and quality of scores for target-decoy separation.

pyprophet export feature-scores

Export feature score plots from a PyProphet input file.

Creates plots showing the distribution of feature scores (var_* columns) at different levels (ms1, ms2, transition, alignment) colored by target/decoy status. Works with OSW, Parquet, and Split Parquet files (scored or unscored).

pyprophet export feature-scores [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet input file (OSW, Parquet, or Split Parquet directory).

--out <outfile>: Output PDF file. If not provided, will be auto-generated based on input filename.

Export Score Plots (Deprecated)

Deprecated since version 3.1: Use pyprophet export feature-scores instead.

The export score-plots command is deprecated and will be removed in a future version. It has been replaced by the more flexible export feature-scores command which works with all file formats.

pyprophet export score-plots

Export score plots (DEPRECATED - use ‘feature-scores’ instead)

This command is deprecated. Please use ‘pyprophet export feature-scores’ instead.

pyprophet export score-plots [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet OSW input file.

--glycoform, --no-glycoform

Export glycoform score plots.

Default:: False

Export Results Report

To export a PDF report of the results, you can use the export score-report subcommand. This is useful for generating a report that summarizes the results of your analysis, including scores and identifications, and other relevant information.

pyprophet export score-report

Export report with scored results from a PyProphet input file.

pyprophet export score-report [OPTIONS]

Options

--helphelp: Show advanced help with all options.

--in <infile>: Required PyProphet OSW input file.

Merge files

PyProphet provides a command to merge multiple files into a single file. This is useful for combining results from different analyses or runs into a single file for further processing or analysis.

Merge OSW Files

To merge multiple OSW files into a single OSW file, you can use the merge osw subcommand.

pyprophet merge osw

Merge multiple OSW files and (for large experiments, it is recommended to subsample first).

pyprophet merge osw [OPTIONS] [INFILES]...

Options

--helphelp: Show advanced help with all options.

--out <outfile>: Required Merged OSW output file.

--same_run, --no-same_run: Assume input files are from same run (deletes run information).

--template <templatefile>: Required Template OSW file.

--merged_post_scored_runs: Merge OSW output files that have already been scored.

Arguments

INFILES: Optional argument(s)

Merge Parquet Files

To merge multiple Parquet files into a single Parquet file, you can use the merge parquet subcommand.

pyprophet merge parquet

Merge multiple parquet files.

pyprophet merge parquet [OPTIONS] [INFILES]...

Options

--helphelp: Show advanced help with all options.

--out <outfile>: Required Merged parquet output file.

--merge_transitions, --no-merge_transitions: If the input is of type split_parquet / split_parquet_multi, merge the separate transition files into a single file as well.

Arguments

INFILES: Optional argument(s)